The city of Bulawayo in Zimbabwe has recorded four suspected cases of the deadly disease with authorities tracing the ailments to Harare.
Reports said on Saturday that three of the patients have been quarantined at the Thorngrove hospital.
Bulawayo had remained a relatively free spot for cholera and other waterborne diseases outbreak,
Harare has however suffered a devastating cholera outbreak that has claimed 27 lives while nearly 4 000 have also been infected.
The high-density residential areas have battled sanitation challenges with drinking water sources reportedly contaminated through recurrent sewer leakages.